Journal: eLife
Article Title: A delta-tubulin/epsilon-tubulin/Ted protein complex is required for centriole architecture
doi: 10.7554/eLife.98704
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Article Snippet: Primary antibodies used for immunofluorescence and U-ExM and dilutions in PBS-BT: mouse IgG2b anti-acetylated-tubulin, clone 6-11B-1 (1:1000,Sigma-Aldrich Cat# T6793, RRID: AB_477585 ), rabbit anti-acetyl-α-tubulin (Lys40) (1:100, Cell Signaling Technology Cat# 5335, RRID: AB_10544694 ), mouse IgG2b anti-centrin3, clone 3e6 (1:1000, Novus Biological, RRID: AB_537701 ), mouse IgG2a anti-centrin, clone 20H5 (IF 1:200, UExM 1:500, EMD Millipore, RRID: AB_10563501 ), rat anti-Cep120 (1:1000, gift from Moe Mahjoub ), rabbit anti-Cep135 (1:500, Proteintech Cat# 24428–1-AP, RRID: AB_2879543 ), rabbit anti-Cep295 (1:1000, Sigma-Aldrich Cat# HPA038596, RRID: AB_10672720 ), rabbit anti-Cep44 (1:100, Proteintech Cat# 24457–1-AP, RRID: AB_2879557 ), rabbit anti-CENPJ (1:500, Proteintech Cat# 11517–1-AP, RRID: AB_2244605 ), rabbit anti-CP110 (IF 1:200, UExM 1:2000, Proteintech Cat# 12780–1-AP, RRID: AB_10638480 ), mouse IgG1 anti-Flag, clone M2 (1:500, Sigma-Aldrich Cat# F1804, RRID: AB_262044 ), mouse IgG1 anti-gamma-tubulin, clone GTU-88 (IF 1:1000, UExM 1:500, Sigma-Aldrich, RRID: AB_477584 ), mouse IgG2a anti-PCNA (1:500, BioLegend, RRID: AB_314692 ), rabbit anti-POC5 (for IF: 1:500, Bethyl Laboratories, RRID: AB_10949152 ), rabbit anti-POC5 (for U-ExM: 1:500, Thermo Fisher Scientific Cat# A303-341A (also A303-341A-T), RRID: AB_10971172 ), mouse IgG1 anti-polyglutamylation, clone GT335 (1:500, AdipoGen Cat# AG-20B-0020, RRID: AB_2490210 ), rabbit anti-polyglutamate-chain, polyE (1:500, AdipoGen Cat# AG-25B-0030, RRID: AB_2490540 ), mouse IgG2b anti-SASS6 (1:200, Santa Cruz Cat# sc-81431, RRID: AB_1128357 ), rabbit anti-STIL (1:500, Abcam Cat# ab89314, RRID: AB_2197878 ), mouse IgG2a anti-V5 (1:00, Thermo Fisher Scientific Cat# R960-25, RRID: AB_2556564 ), rabbit anti-WDR90 (1:100, Thermo Fisher Scientific Cat# PA5-61943, RRID: AB_2649628 ), chicken anti-GFP antibody (Aves Cat# GFP-1020, RRID: AB_10000240 ).
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